Computer based exercises in Molecular Cloning Strategies

Try an exercise @ https://sites.google.com/site/thednalab/tryanexercise

My email: siddharth@inspirelabs.org

Computer based exercises in Molecular Cloning Strategies

It’s a set of nine exercises where you can simulate cloning and gene expression experiments in computer. Each exercise comprises of-

1. A PDF that describes the questions and explains answer as text

2. GenBank files of molecules discussed in the question

3. A detailed video answer that takes you step-by-step and click-by-click through the entire solution on SerialCloner software (One can download SerialCloner free from http://serialbasics.free.fr/Serial_Cloner.html)

Who can use “Computer based exercises in Molecular Cloning Strategies?”

1. Students who have read gene transcription/ translation from textbooks. They will find it exciting to perform in silico (in computer) experiments and put their knowledge to test. Students will also become skilled in developing cloning strategies using software.

2. Research laboratories, which often perform cloning experiments. Researchers of the lab will get skilled in developing cloning strategies using software. Researchers can store/organize entire cloning experiments, strategies and new molecules in computer.

3. Any cloning enthusiast is welcome to try it!

What is included in this set?

• Nine exercises -
  

1. Sub-clone “gene X” from vector pTDL10 into cloning plasmid pBR322

2. Directional cloning, Sub-clone human erythropoietin gene from plasmid pTDL14 into expression vector pGEX-5X3

3. Directional cloning, using reverse complement function

4. Directional cloning with compatible cohesive ends

5. PCR based cloning, Clone GeneY from pTDL11 into cloning plasmid pUC19

6. PCR based cloning, Clone Gene BBK56 into expression vector plasmid bbk56 into pGEX3X

7. Clone GFP into pUC19

8. Inframe cloning, Express human erythropoietin gene as fusion protein

9. Adjust reading frame, Subclone BKK56 gene in expression vector pGEX5X3

• Molecules as GenBank file: pGEX-5X3, pTDL11, GFP aequorea Victoria, pUC19, pTDL 13, pTDL 14, pET-50b(+), pTDL12, Human erythropoietin in pACYC177, pMAL-C2T, pGEX3X, pBR322, pTDL10
• Video answers for all nine exercises